Our platform now provides interactive 3D material or biological structure in real-time to enhance high-throughput specimen interpretation. Here we present facile 3D visualization of specimens during an electron or cryo-electron tomography experiment using the tomviz platform (). It has been a longstanding goal to begin 3D analysis of specimens in real time to allow immediate assessment of nanoscale structure and data quality 9. While advancements in detector hardware have boosted throughput with digital data collection 8, substantial human effort and computational resources are still required to process the raw data before visualization. Even worse, the reconstruction occurs offline, long after all the data has been collected, preventing immediate interpretation during an ongoing experiment. Unfortunately, tomographic reconstructions can take one to several days to complete depending upon the dataset size or algorithm(s) employed. In an electron tomography experiment, the volumetric structure of biological or material specimens is reconstructed from high-resolution projection images acquired across many viewing angles 6, 7. Three-dimensional (3D) characterization across the nanoscale is now possible using scanning/transmission electron microscopes (S/TEM) 1– 5.
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